Catalogue number
PRO-2257
Synonyms
V-CAM
1, VCAM-1, CD106, Vcam1, Vcam-1, Vascular cell adhesion protein 1.
Introduction
VCAM1 is a member of the Ig superfamily. It is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. VCAM-1 contains 6 or 7 immunoglobulin domains, and is expressed on both large and small vessels only after the endothelial cells are stimulated by cytokines. The protein has a number of functions including the regulation of leukocyte migration, leukocyte endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases (artherosclerosis and rheumatoid arthritis).
Description
VCAM1 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated? polypeptide chain containing 682 amino acids (25-698 a.a) and having a molecular mass of 75.4kDa (Migrates at 70-100kDa on SDS-PAGE under reducing conditions). VCAM1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
VCAM1 protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10%
glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
FKIEISPEYK TIAQIGDSMA LTCSTTGCES PLFSWRTQID SPLNAKVRTE
GSKSVLTMEP VSFENEHSYL CTATCGSGKL ERSIHVDIYS FPKDPEIQFS GPLEVGKPVT VKCLAPDIYP
VYRLEIDLFK GDQLMNRQEF SSEEMTKSLE TKSLEVTFTP VIEDIGKALV CRAKLHIDQI DSTLKERETV
KELQVYISPR NTTISVHPST RLQEGGAVTM TCSSEGLPAP EIFWGRKLDN EVLQLLSGNA TLTLIAMRME
DSGVYVCEGV NLIGRDKAEV ELVVQEKPFI VDISPGSQVA AQVGDSVVLT CAAIGCDSPS FSWRTQTDSP LNGVVRNEGA KSTLVLSSVG
FEDEHSYLCA VTCLQRTLEK RTQVEVYSFP EDPVIKMSGP LVHGRPVTVN CTVPNVYPFD HLEIELLKGE
TTLMKKYFLE EMGIKSLETK ILETTFIPTI EDTGKSLVCL ARLHSGEMES EPKQRQSVQP LYVNVAPKET
TIWVSPSPIL EEGSPVNLTC SSDGIPAPKI LWSRQLNNGE LQPLSENTTL TFMSTKRDDS GIYVCEGINE
AGISRKSVEL IIQVSPKDIQ LTVFPSKSVK EGDTVIISCT CGNVPETWII LKKKAKTGDM
VLKSVDGSYT IRQAQLQDAG IYECESKTEV GSQLRSLTLD VKGKEHNKNY FSPELEHHHH HH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
