Catalogue number
PRO-1905
Synonyms
FRP-2, SARP1, SDF-5, Secreted frizzled-related protein 2,
UNQ361/PRO697, FKSG12, FKSG12.
Introduction
Secreted frizzled-related
protein 2 (SFRP2) belongs to the SFRP family which contains a cysteine-rich
domain homologous to the putative Wnt- of Frizzled proteins. SFRP2
function as soluble modulators of Wnt signaling. Methylation of SFRP2 is a
possible marker for the existence of colorectal cancer.
Description
SFRP2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 297 amino acids (20-295 a.a) and having a molecular mass of 33.8kDa.
SFRP2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SFRP2 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSARGLFLF GQPDFSYKRS NCKPIPVNLQ LCHGIEYQNM RLPNLLGHET MKEVLEQAGA
WIPLVMKQCH PDTKKFLCSL FAPVCLDDLD ETIQPCHSLC VQVKDRCAPV MSAFGFPWPD MLECDRFPQD
NDLCIPLASS DHLLPATEEA PKVCEACKNK NDDDNDIMET LCKNDFALKI KVKEITYINR DTKIILETKS KTIYKLNGVS
ERDLKKSVLW LKDSLQCTCE EMNDINAPYL VMGQKQGGEL VITSVKRWQK GQREFKRISR SIRKLQC.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
