Catalogue number
PRO-1110
Synonyms
Signal-transducing adaptor protein 1, STAP-1, BCR downstream-signaling protein 1, Docking protein BRDG1, Stem cell adaptor protein 1, STAP1, BRDG1.
Introduction
Signal-transducing adaptor protein 1 (STAP1) acts as a docking protein which operates downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The STAP1 protein is directly phosphorylated by Tec in vitro where it partakes in a positive feedback loop, increasing Tec activity.
Description
STAP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 319 amino acids (1-295 a.a) and having a molecular mass of 36.8kDa.
STAP1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
STAP1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1M NaCl and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSHMMAKKP PKPAPRRIFQ ERLKITALPL YFEGFLLIKR SGYREYEHYW TELRGTTLFF YTDKKSIIYV DKLDIVDLTC LTEQNSTEKN CAKFTLVLPK EEVQLKTENT ESGEEWRGFI LTVTELSVPQ NVSLLPGQVI KLHEVLEREK KRRIETEQST SVEKEKEPTE DYVDVLNPMP ACFYTVSRKE ATEMLQKNPS LGNMILRPGS DSRNYSITIR QEIDIPRIKH YKVMSVGQNY TIELEKPVTL PNLFSVIDYF VKETRGNLRP FICSTDENTG QEPSMEGRSE KLKKNPHIA.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
