Catalogue number
PRO-2326
Synonyms
LAB7,
CD28LG, CD28LG1, CD28LG1, B71.
Introduction
The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells CD-80 is involved in the costimulatory signal essential for t- lymphocyte activation. t-cell proliferation and cytokine production is induced by the binding of cd28 or ctla-4 to this receptor.
Description
CD80 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 216 amino acids (37-246 a.a.) and
having a molecular mass of 24.7kDa (Migrates at 28-40kDa on SDS-PAGE under
reducing conditions).
CD80 is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
CD80 protein solution (0.25mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.?
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).?
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
DVDEQLSKSV KDKVLLPCRY NSPHEDESED RIYWQKHDKV VLSVIAGKLK
VWPEYKNRTL YDNTTYSLII LGLVLSDRGT YSCVVQKKER GTYEVKHLAL VKLSIKADFS TPNITESGNP
SADTKRITCF ASGGFPKPRF SWLENGRELP GINTTISQDP ESELYTISSQ LDFNTTRNHT IKCLIKYGDA
HVSEDFTWEK PPEDPPDSKN HHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
