Catalogue number
CYT-664
Synonyms
Scatter Factor, SF, Hepatopoietin, HPTA, HGF, HGFB, F-TCF, DFNB39, Hepatocyte growth factor, Hepatopoeitin-A, Hepatocyte growth factor alpha chain.
Introduction
Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. HGF is secreted as a single inactive polypeptide which is cleaved by serine proteases into a 69kDa Alpha chain and 34kDa Beta chain. A disulfide bond linking the alpha and beta chains produces the active, heterodimeric molecule.
Description
HGF-A Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 463 amino acids fragment (32-494) having a total molecular weight of 57.8kDa. The HGF-A is fused with a 4.5kDa amino-terminal hexahistidine tag.
The HGF-A is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
HGF-A protein is supplied in 25mM Na. Acetate pH4.8, 1mM EDTA and 50% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCAN
RCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKK
EFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQP
WSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWC
FTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTES
GKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQ
PRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTEC
IQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPEN
FKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNC
DMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNME
DLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIP
WDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLR.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
