Catalogue number
ANT-524
Synonyms
Coactosin-like protein, COTL1, CLP, FLJ43657, MGC19733.
Type
Mouse Anti Human Monoclonal.
Introduction
Coactosin-like protein (COTL1) is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. COTL1 binds F-actin, it also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. COTL1 binds to F-actin in a calcium-independent way and has no direct effect on actin depolymerization.
Clone
PAT1D6AT.
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human COTL1 mAb,
clone PAT1D6AT, is derived from hybridization of mouse F0 myeloma cells with
spleen cells from BALB/c mice immunized with a recombinant human COTL1 protein 1-142
amino acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and k light chain.
Purification Method
COTL1 antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.?
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
The antibody has been tested by ELISA, Western blot
analysis to assure specificity and reactivity. Since application varies,
however, each investigation should be titrated by the reagent to obtain optimal
results. Recommended starting dilution is 1:1000.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
