Catalogue number
ANT-519
Synonyms
Creatine kinase mitochondrial 1A, creatine kinase
mitochondrial 1 (ubiquitous), creatine kinase U-type mitochondrial, Acidic-type
mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, CKMT1,
U-MtCK, mia-CK, EC 2.7.3, EC 2.7.3.2.
Type
Mouse Anti Human Monoclonal.
Introduction
CKMT1A is in charge of the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. CKMT1A is a member of the creatine kinase isoenzyme family and exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase arises in two different oligomeric forms: dimers and octamers, unlike the exclusively dimeric cytosolic creatine kinase isoenzymes. Numerous malignant cancers with poor prognosis have displayed overexpression of ubiquitous mitochondrial creatine kinase which is linked to high energy turnover and inability to remove cancer cells through apoptosis.
Clone
PAT17A2AT.
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human CKMT1A mAb,
clone PAT17A2AT, is derived from hybridization of mouse F0 myeloma cells with
spleen cells from BALB/c mice immunized with a recombinant human CKMT1A protein
40-417 amino acids purified from E.
coli.
Ig Subclass
Mouse
IgG2b heavy chain and k light chain.
Purification Method
CKMT1A antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.?
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
The antibody has been tested by ELISA, Western blot
analysis to assure specificity and reactivity. Since application varies,
however, each investigation should be titrated by the reagent to obtain optimal
results. Recommended starting dilution is 1:1000.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
