Catalogue number
PRO-2097
Synonyms
Ribosomal Protein L35, 60S Ribosomal Protein L35, RPL35A,
DBA5, L35, 60S ribosomal protein L35.
Introduction
Ribosomal
Protein L35, also known as RPL35 is a ribosomal protein which is a component of
the 60S subunit. Ribosomes are the organelles which catalyze protein synthesis,
they consist of a small 40S subunit and a large 60S subunit. Jointly these
subunits are composed of 4 RNA species and about 80 structurally different
proteins. RPL35
is a member of? the L29P family of
ribosomal proteins. RPL35 is located in the cytoplasm. As typicaly for genes
encoding ribosomal proteins, there are multiple processed pseudogenes of RPL35
dispersed through the genome.
Description
RPL35 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 146 amino acids (1-123 a.a) and
having a molecular mass of 16.9kDa.
RPL35 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
RPL35 protein solution (0.25 mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAKIKAR DLRGKKKEEL LKQLDDLKVE LSQLRVAKVT GGAASKLSKI
RVVRKSIARV LTVINQTQKE NLRKFYKGKK YKPLDLRPKK TRAMRRRLNK HEENLKTKKQ QRKERLYPLR
KYAVKA.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
