Catalogue number
PRO-2382
Synonyms
Retinoic
Acid Receptor Responder 2, Chemerin, Retinoic Acid Receptor Responder
(Tazarotene Induced) 2, Tazarotene-Induced Gene 2 Protein, RAR-Responsive
Protein TIG2, TIG2, Retinoic Acid Receptor Responder Protein 2, HP10433, Retinoic
acid receptor responder protein 2, Chemerin, RAR-responsive protein TIG2, Tazarotene-induced
gene 2 protein.
Introduction
RARRES2 is a secreted chemotactic protein that initiates chemotaxis through the ChemR23 G protein-coupled seven-transmembrane domain ligand. RARRES2 is upregulated by the synthetic retinoid tazarotene and found in a vast variety of tissues. RARRES2 acts as an adipokine, and is truncated on both termini from the proprotein. RARRES2 is structurally related to the cathelicidin precursors, cystatin C and kininogens. RARRES2 promotes calcium mobilization and chemotaxis of immature dendritic cells and macrophages. RARRES2 is secreted as a precursor of little biological activity, which requires proteolytic cleavage of its COOH-terminal domain to be exchangeed into a potent and highly specific agonist of ChemR23. RARRES2 signals via its receptor, ChemR23 (CMKLR1), as a positive regulator of adipocyte differentiation and metabolic function. The Chemerin receptor acts as a coreceptor for SIV and some primary HIV-1 strains. The Chemerin receptor has another ligand, called tazarotene-induced gene.
Description
RARRES2 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 146 amino acids (21-157a.a.) and
having a molecular mass of 16.9kDa. (Molecular size on SDS-PAGE will appear at
approximately 18-28kDa).
RARRES2 is expressed with a
6 amino acid His tag at C-Terminus and purified by proprietary chromatographic
techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
RARRES2
protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10%
glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
ADPELTEAQR RGLQVALEEF
HKHPPVQWAF QETSVESAVD TPFPAGIFVR LEFKLQQTSC RKRDWKKPEC KVRPNGRKRK CLACIKLGSE DKVLGRLVHC
PIETQVLREA EEHQETQCLR VQRAGEDPHS FYFPGQFAFS HHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
