Catalogue number
PRO-2174
Synonyms
Transcription factor PU.1, 31 kDa-transforming protein, Transcription factor PU.1 isoform 1, SPI1, OF, PU.1, SFPI1, SPI-1, SPI-A.
Introduction
Spi-1
Proto-Oncogene, also known as SPI1, owns ETS-domain transcription factor which
activates gene expression during myeloid and Blymphoid cell development. SPI1 binds
to a purine-rich sequence called the PU-box which is located near the promoters
of target genes, and regulates their expression. SPI1 binds RNA and modulate
pre-mRNA splicing. SPI1 is also regulates alternative splicing of target
genes.
Description
SPI1 Human Recombinant produced in E. coli is a single polypeptide chain containing 294 amino acids (1-271) and having a molecular mass of 33.6kDa.
SPI1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The SPI1 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMLQACKM EGFPLVPPQP SEDLVPYDTD LYQRQTHEYY PYLSSDGESH SDHYWDFHPH HVHSEFESFA ENNFTELQSV QPPQLQQLYR HMELEQMHVL DTPMVPPHPS LGHQVSYLPR MCLQYPSLSP AQPSSDEEEG ERQSPPLEVS DGEADGLEPG PGLLPGETGS KKKIRLYQFL LDLLRSGDMK DSIWWVDKDK GTFQFSSKHK EALAHRWGIQ KGNRKKMTYQ KMARALRNYG KTGEVKKVKK KLTYQFSGEV LGRGGLAERR HPPH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
