Catalogue number
PRO-1913
Synonyms
Suppressor Of IKBKE 1, SIKE, Suppressor Of IKK Epsilon, Suppressor
Of IKK-Epsilon.
Introduction
Suppressor of IKBKE 1 also known as SIKE1 belongs to the SIKE family. SIKE interacts with IKK-epsilon and TBK1 and function as a suppressor of TLR3. In addition, SIKE1 perform by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 andDDX58/RIG-I. SIKE1 does not inhibit NF-kappa-B activation pathways.
Description
SIKE1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 230 amino acids (1-207 a.a) and
having a molecular mass of 26.1kDa.
SIKE1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SIKE1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30%
glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSCTIEK ILTDAKTLLE RLREHDAAAE SLVDQSAALH RRVAAMREAG
TALPDQYQED ASDMKDMSKY KPHILLSQEN TQIRDLQQEN RELWISLEEH QDALELIMSK YRKQMLQLMV
AKKAVDAEPV LKAHQSHSAE IESQIDRICE MGEVMRKAVQ VDDDQFCKIQ EKLAQLELEN KELRELLSIS SESLQARKEN SMDTASQAIK
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
