Catalogue number
PRO-1698
Synonyms
Protein sprouty homolog 4, SPRY4, Sprouty Homolog 4, Spry-4, Protein sprouty homolog 4 isoform 2, Sprouty homolog 4 (Drosophila).
Introduction
Sprouty Homolog 4 (SPRY4) is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. SPRY4 is positioned upstream of RAS activation. SPRY4 protein functions include suppressing?the EGFR-transduced MAPK signaling pathway as well as impairing the formation of GTP-Ras.
Description
SPRY4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 322 amino acids (1-299) and having a molecular mass of 34.9 kDa.
SPRY4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The SPRY4 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMEPPIPQ SAPLTPNSVM VQPLLDSRMS HSRLQHPLTI LPIDQVKTSH VENDYIDNPS LALTTGPKRT RGGAPELAPT PARCDQDVTH HWISFSGRPS SVSSSSSTSS DQRLLDHMAP PPVADQASPR AVRIQPKVVH CQPLDLKGPA VPPELDKHFL LCEACGKCKC KECASPRTLP SCWVCNQECL CSAQTLVNYG TCMCLVQGIF YHCTNEDDEG SCADHPCSCS RSNCCARWSF MGALSVVLPC LLCYLPATGC VKLAQRGYDR LRRPGCRCKH TNSVICKAAS GDAKTSRPDK PF.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
