Catalogue number
CYT-789
Synonyms
Neuritin 1, NRN1, NRN, dJ380B8.2, Neuritin.
Introduction
Neuritin (NRN1) is a neurotrophic factor, which is expressed in response to induction of neuronal activity by NGF, BDNF, NT3 and other neural stimulators. NRN1 is expressed principally in postmitotic-differentiating neurons of the developing nervous system and in neuronal structures related to synaptic plasticity in the ***** nervous system. NRN1 functions as a molecular mediator of neurite outgrowth, neuronal survival, and synaptic maturation.
Description
Recombinant Human NRN1 produced in E.coli cells is a non-glycosylated, homodimeric protein containing 2x88 amino acid chains and having a molecular mass of 19.4kDa.
The NRN1 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The NRN1 was lyophilized from a 0.2μm filtered concentrated solution in 1×PBS, pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized NRN1 in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized NRN1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NRN1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
AGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGN.
Biological Activity
The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 25ng/ml, corresponding to a specific activity of > 4.0 × 104 IU/mg.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
