Catalogue number
PRO-1518
Synonyms
Neuromedin-B, NMB, Neuromedin-B-32, Neuromedin B.
Introduction
Neuromedin B (NMB) which is a part of the bombesin/neuromedin-B/ranatensin family and stimulates smooth muscle contraction in a way similar to that of bombesin.
Description
NMB Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 120 amino acids (25-121) and having a molecular mass of 13.2 kDa.
NMB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The NMB solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSAPLSWDL PEPRSRASKI RVHSRGNLWA TGHFMGKKSL EPSSPSPLGT APHTSLRDQR LQLSHDLLGI LLLKKALGVS LSRPAPQIQY RRLLVQILQK.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
