Catalogue number
PRO-1835
Synonyms
ChaC Cation Transport Regulator Homolog 2 (E. Coli), ChaC Cation Transport Regulator-Like 2 (E. Coli), Gamma-GCT Acting On Glutathione Homolog 2.
Introduction
Cation transport regulator-like protein 2 (CHAC2) is a member of the chaC family.?Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides.
Description
CHAC2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 207 amino acids (1-184) and having a molecular mass of 23.3 kDa. CHAC2 is fused to a 23 amino acid His-tag at N-terminus.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The CHAC2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 100mM NaCl and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMWVFGYG SLIWKVDFPY QDKLVGYITN YSRRFWQGST DHRGVPGKPG RVVTLVEDPA GCVWGVAYRL PVGKEEEVKA YLDFREKGGY RTTTVIFYPK DPTTKPFSVL LYIGTCDNPD YLGPAPLEDI AEQIFNAAGP SGRNTEYLFE LANSIRNLVP EEADEHLFAL EKLVKERLEG KQNLNCI
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
