Catalogue number
PRO-1492
Synonyms
BUD31 homolog (S. cerevisiae), Cwc14, EDG-2, EDG2, fSAP17, G10, YCR063W, Protein G10 homolog.
Introduction
BUD31 is a member of the BUD31 (G10) family. BUD31 is a nuclear protein which contains a putative nuclear translocation sequence, an N-terminal acidic domain and a cysteine-rich C-terminal domain containing a putative Zinc-finger structure. Based on the structure of BUD31 it is suggested that BUD31 might be a nuclear regulator of transcription.
Description
BUD31 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-144 a.a.) and having a molecular mass of 19.4kDa.
BUD31 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
BUD31 protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer, (pH 8.0), 0.2M NaCl, 40% glycerol and 2mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMPKVKRS RKAPPDGWEL IEPTLDELDQ KMREAETEPH EGKRKVESLW PIFRIHHQKT RYIFDLFYKR KAISRELYEY CIKEGYADKN LIAKWKKQGY ENLCCLRCIQ TRDTNFGTNC ICRVPKSKLE VGRIIECTHC GCRGCSG.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
