Catalogue number
PRO-2075
Synonyms
Neuronal Calcium Sensor 1, Frequenin-Like Ubiquitous
Protein, Frequenin-Like Protein, Frequenin Homolog, FREQ, FLUP, Frequenin
(Drosophila) Homolog, Frequenin Homolog (Drosophila), FREQ, NCS-1, Neuronal
calcium sensor 1.
Introduction
NCS1 is part of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed primarily in neurons. NCS1 regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. NCS1 is related with secretory granules and modulates synaptic transmission and synaptic plasticity. NCS1 regulates GRK1 and substitutes for calmodulin. NCS1 stimulates PI4KB kinase activity and participates in long-term synaptic plasticity through its interaction with PICK1. NCS1 takes part in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.
Description
NCS1 213 a.a. Human Recombinant produced in E.Coli is a
single, non-glycosylated polypeptide chain containing 213 amino acids (1-190 a.a)
and having a molecular mass of 24.3kDa.
NCS1 213 a.a. is fused to a 23 amino acid His-tag at
N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear colorless solution.
Formulation
NCS1 213 a.a. protein solution (1mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.15M NaCl,
30% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMGKSNSK LKPEVVEELT RKTYFTEKEV QQWYKGFIKD CPSGQLDAAG
FQKIYKQFFP FGDPTKFATF VFNVFDENKD GRIEFSEFIQ ALSVTSRGTL DEKLRWAFKL YDLDNDGYIT
RNEMLDIVDA IYQMVGNTVE LPEEENTPEK RVDRIFAMMD
KNADGKLTLQ EFQEGSKADP SIVQALSLYD GLV.
Safety Data Sheet
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
