Catalogue number
PRO-2186
Synonyms
Kruppel-Like
Factor 4 (Gut), GKLF, EZF, Epithelial Zinc Finger Protein EZF, Gut-Enriched
Krueppel-Like Factor, Endothelial Kruppel-Like Zinc Finger Protein,
Krueppel-Like Factor 4, Krueppel-like factor 4, Epithelial zinc finger protein
EZF, Gut-enriched krueppel-like factor.
Introduction
KLF4 is a transcription factor that performs as both an activator and repressor. KLF4 is expressed mainly in erythroid tissues and found mostly in gut. KLF4 is takes part in the differentiation of epithelial cells in addition to skeletal and kidney development.
Description
KLF4 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 422 amino acids (11-395 a.a) and
having a molecular mass of 44.2kDa.
KLF4 is fused to a 37 amino
acid His-tag at N-terminus & purified by proprietary chromatographic
techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
KLF4 protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAVS DALLPSFSTF ASGPAGREKT LRQAGAPNNR WREELSHMKR
LPPVLPGRPY DLAAATVATD LESGGAGAAC GGSNLAPLPR RETEEFNDLL DLDFILSNSL THPPESVAAT
VSSSASASSS SSPSSSGPAS APSTCSFTYP IRAGNDPGVA PGGTGGGLLY GRESAPPPTA PFNLADINDV
SPSGGFVAEL LRPELDPVYI PPQQPQPPGG GLMGKFVLKA SLSAPGSEYG SPSVISVSKG SPDGSHPVVV
APYNGGPPRT CPKIKQEAVS SCTHLGAGPP LSNGHRPAAH DFPLGRQLPS RTTPTLGLEE VLSSRDCHPA
LPLPPGFHPH PGPNYPSFLP DQMQPQVPPL HYQELMPPGS CMPEEPKPKR GRRSWPRKRT AT.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
