Catalogue number
PRO-1086
Synonyms
Ig gamma-2A chain C region, A allele, Immunoglobulin heavy chain gamma polypeptide, Ighg, Igh-1, Igh-1a, 1810060O09Rik.
Introduction
Immunoglobulin G (IgG)?are antibody molecules. Each IgG is composed of four peptide chains?- 2 heavy chains?g and 2 light chains. Also, each IgG has 2 antigen s. IgG antibodies are involved in primarily the secondary immune response. The presence of specific IgG, generally, relates to maturation of the antibody response. IgG also has an imperative role in Antibody-dependent cell-mediated cytotoxicity (ADCC) and Intracellular antibody-mediated proteolysis, in which it binds to TRIM21 (the receptor with greatest affinity to IgG in humans) in order to direct marked virions to the proteasome in the cytosol.
Description
IgG Mouse Recombinant produced in Hi-5 cells is a single polypeptide chain containing 242 amino acids (98-330 a.a) and having a molecular mass of 27.5kDa.
IgG is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Source
Hi-5 cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
IgG protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
ADPEPRGPTI KPCPPCKCPA PNLLGGPSVF IFPPKIKDVL MISLSPIVTC VVVDVSEDDP DVQISWFVNN VEVHTAQTQT HREDYNSTLR VVSALPIQHQ DWMSGKEFKC KVNNKDLPAP IERTISKPKG SVRAPQVYVL PPPEEEMTKK QVTLTCMVTD FMPEDIYVEW TNNGKTELNY
KNTEPVLDSD GSYFMYSKLR VEKKNWVERN SYSCSVVHEG LHNHHTTKSF SRTPGKHHHH HH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
