Catalogue number
HSP-032
Synonyms
cvHSP, DKFZp779D0968, FLJ32733, HSPB7, CVHSP.
Introduction
HSPB7 is part of the Heat shock protein beta family. The HSPB family is one of the more diverse families within the group of HSP families. Several members have chaperone-like activities and/or participate in cytoskeletal stabilization. Several members also show a dynamic, stress-induced translocation to SC35 splicing speckles. HSPB7 constitutively localized to SC35 splicing speckles, determined by its N-terminus. HSPB7 does not support refolding though HSPB1 and HSPB5 chaperone heat unfolded substrates and keet them folding competent.
Description
HSPB7 Human Recombinant produced in E.Coli is a single, non-glycosylated,
polypeptide chain containing 190 amino acids (1-170 a.a.) and having a molecular mass of 20.7 kDa. HSPB7 protein is fused to a 20 amino acid His-Tag at N-terminus and purified by standard chromatography.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
HSPB7 Human solution containing 20mM Tris HCl pH-8, 0.2M NaCl, 2mM DTT & 50% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MSHRTSSTFR AERSFHSSSS SSSSSTSSSA SRALPAQDPP MEKALSMFSD DFGSFMRPHS EPLAFPARPG GAGNIKTLGD AYEFAVDVRD FSPEDIIVTT SNNHIEVRAE KLAADGTVMN TFAHKCQLPE DVDPTSVTSA LREDGSLTIR ARRHPHTEHV QQTFRTEIKI.
Safety Data Sheet
Usage
ProSpec's products are furnished for?LABORATORY RESEARCH?USE?ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
