Catalogue number
PRO-2165
Synonyms
C-type lectin domain family 5 member A , CLECSF5, MDL-1,
MDL1, C-type lectin superfamily member 5, Myeloid DAP12-associating lectin 1.
Introduction
C-type lectin domain family 5-member A isoform 1 (CLEC5A) is part of the CTL/CTLD superfamily which carry various functions, for instance cell-cell signaling, cell adhesion, glycoprotein turnover, in addition to their inflammation & immune response abilities. CLEC5A operates as a cell attachment receptor for all 4 serotypes of Dengue virus in addition to Japanese encephalitis virus. CLEC5A binds to the dengue virus and it triggers signaling through the phosphylation of TYROBP, as a result no viral entrance occurs, however this interaction does stimulate proinflammatory cytokine release. CLEC5A preforms as a positive regulator of osteoclastogenesis and also a main regulator of synovial injury & bone erosion for the period of autoimmune joint inflammation.
Description
CLEC5A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 184 amino acids (28-188 a.a) and having a molecular mass of 20.8kDa.
CLEC5A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
CLEC5A protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer, (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSPQIFNKS NDGFTTTRSY GTVSQIFGSS SPSPNGFITT RSYGTVCPKD WEFYQARCFF LSTSESSWNE SRDFCKGKGS TLAIVNTPEK LKFLQDITDA EKYFIGLIYH REEKRWRWIN NSVFNGNVTN QNQNFNCATI GLTKTFDAAS CDISYRRICE KNAK.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
