Catalogue number
ANT-342
Synonyms
Calmodulin, CaM, CALM2, PHKD, CAMII, PHKD2, calmodulin 2, phosphorylase kinase delta.
Type
Mouse Anti Human Monoclonal.
Introduction
Calmodulin (CaM) is an intracellular receptor protein for Ca2+-ions. It activates the myosin light chain kinase which is the catalyst of myosin phosphorylation. CaM participates in the activation of enzymes such as cyclic nucleotide- dependent phosphodiesterase, calcineurin, ATPase, Myosin Light Chain Kinases, and CAM kinase.
Clone
PJ4D8AT.
Immunogen
Anti-human Calmodulin mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Calmodulin amino acids 1-149 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and κ light chain.
Purification Method
Calmodulin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
Calmodulin antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
