Catalogue number
ANT-145
Synonyms
gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4, CD4mut.
Type
Mouse Anti Human Monoclonal.
Introduction
CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cell with either have CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized.
Clone
hCD4.
Immunogen
Purified human PBL CD4+ T cells.
Ig Subclass
Mouse IgG2b.
Purification Method
Ion exchange column.
Formulation
1mg/ml in PBS (after reconstitution).
Storage Procedures
Lyophilized: store at 4oC. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.
Solubility
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Applications
Blocking and staining antibody. Blocks blinding of HIV to CD4. For staining, use 10 μl/1,000,000 cells. Titer for blocking T cell activation should be determined by the investigator.
Available Conjugates
This antibody is also available conjugated to biotin and FITC.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
