Catalogue number
PRO-111
Synonyms
60 kDa SS-A/Ro ribonucleoprotein, 60 kDa ribonucleoprotein Ro, RoRNP, 60 kDa Ro protein, Ro 60 kDa autoantigen, TROVE domain family member 2, Sjoegren syndrome type A antigen, SS-A, Sjoegren syndrome antigen A2, TROVE2, RO60, SSA2, RO-60.
Introduction
Ro 60 kDa autoantigen is an RNA-binding protein that binds to several small cytoplasmic RNA molecules known as Y RNAs. SSA2 may stabilize these RNAs from degradation. Sera taken from patients with Systemic Lupus Erythematosus (SLE) often contains antibodies that react with the normal cellular SSA2 protein as if this antigen was foreign.
Description
TROVE2 is a natural protein having a molecular mass of 60kDa. TROVE2 is purified from bovine tissues by protein-chemical methods.
Source
Bovine tissues.
Formulation
TROVE2 is supplied in 20mM Tris/HCl buffer pH-7.5, 150mM NaCl, 2mM MgCl2, 0.2mM DTT, 0.1mM PMSF and 20% glycerol.
Immunological functions
1.?Binds IgG-type human auto-antibodies.
2.?Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.
Applications
Western blot with SLE sera (secondary Sjogren’s syndrome).
Coating concentration
0.2-0.6 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Purity
Greater than 95% as determined by SDS-PAGE.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.?
Avoid multiple freeze-thaw cycles.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
