Catalogue number
PRO-124
Synonyms
Transgelin-2, SM22-alpha homolog, TAGLN2, KIAA0120, HA1756.
Introduction
Transgelin 2 (TAGLN2) is one of the earliest markers of differentiated smooth muscle. TAGLN2 contains a calponin like repeat and a calponin-homology (CH) domain. TAGLN2 is downregulated in some transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. TAGLN2 also binds actin, causing actin fibers to gel within minutes of binding. The binding of transgelin to actin occurs at a ratio of 1:6 actin monomers.
Description
TAGLN2 Human Recombinant fused with a?21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing?208 amino acids (13-199 a.a.) and having a molecular mass of 23.4kDa. The TAGLN2 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The TAGLN2 solution (1 mg/ml), 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MEVQQKIEKQ YDADLEQILI QWITTQCRKD VGRPQPGREN FQNWLKDGTV LCELINALYP EGQAPVKKIQ ASTMAFKQME QISQFLQAAE RYGINTTDIF QTVDLWEGKN MACVQRTLMN LGGLAVARDD GLFSGDPNWF PKKSKENPRN FSDNQLQEGK NVIGLQMGTN RGASQAGMTG YGMPRQIL.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
