Catalogue number
PRO-1832
Synonyms
BROVCA4, R51H3, RAD51L3, TRAD, DNA repair protein RAD51 homolog 4, RAD51 homolog D, RAD51-like protein 3, RAD51D.
Introduction
RAD51D is a member of the RAD51 protein family whose members are extremely similar to bacterial RecA and Saccharomyces cerevisiae Rad51. RAD51 protein family members take part in the homologous recombination and repair of DNA. RAD51D forms a complex with several other members of the RAD51 family which catalyzes homologous pairing between single- and double-stranded DNA, and participates in the early stage of recombinational repair of DNA.
Description
RAD51D Human Recombinant produced in E. coli is. a single polypeptide chain containing 239 amino acids (1-216 a.a) and having a molecular mass of 25.7kDa. RAD51D is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
RAD51D solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMGVLRVG LCPGLTEEMI QLLRSHRIKT VVDLVSADLE EVAQKCGLSY KAEALRRIQV VHAFDIFQML DVLQELRGTV AQQVTGSSGT VKVVVVDSVT AVVSPLLGGQ QREGLALMMQ LARELKTLAR DLGMAVVVTN HITRDRDSGR LKPALGRSWS FVPSTRILLD TIEGAGASGG RRMACLAKSS RQPTGFQEMV DIGTWGTSEQ SATLQGDQT.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
