Catalogue number
PRO-2015
Synonyms
Ribonuclease P protein subunit p20, RNaseP protein p20, Ribonucleases P/MRP protein subunit POP7 homolog, hPOP7, POP7, RPP20, 0610037N12Rik, RPP2.
Introduction
Processing Of Precursor 7, also known as POP7, is a part of the histone-like Alba family. POP7 is a part of ribonuclease P which is a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. POP7 is also a part of RNase MRP complex, which cleaves pre-rRNA sequences.
Description
POP7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids (1-140 a.a.) and having a molecular mass of 18.3kDa.
POP7 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
POP7 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 50% glycerol, 2mM DTT and 2mM EDTA.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMAENR EPRGAVEAEL DPVEYTLRKR LPSRLPRRPN DIYVNMKTDF KAQLARCQKL LDGGARGQNA CSEIYIHGLG LAINRAINIA LQLQAGSFGS LQVAANTSTV ELVDELEPET DTREPLTRIR NNSAIHIRVF RVTPK.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
