Catalogue number
CYT-560
Synonyms
HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b.
Introduction
FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors.
Description
FGF-2 Bovine purified from bovine pituitary is a single, glycosylated, polypeptide chain having a molecular mass of 16kDa.
The basic-FGF is purified by proprietary chromatographic techniques.
Source
Bovine Pituitary.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The FGF-basic Bovine was lyophilized from a concentrated sterile solution containing 50mM Na2HPO4, pH-7.5 & 0.5% HSA.
Solubility
It is recommended to reconstitute the lyophilized FGF-2 Bovine in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized FGF2 Bovine although stable at room temperature for 3 weeks, should be stored desiccated below -18°C.
Upon reconstitution FGF-b Bovine should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
The recommended concentration in responsive cells is 0.1 to 2ng/ml.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
