Catalogue number
CYT-791
Synonyms
Erythropoietin, EP, INN=Epoetin, EPO a, Erythropoietin-alpha,
EPO.
Introduction
EPO a is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. EPO a is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. EPO a also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Description
EPO a Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 189 amino acids (28-193a.a.) and having a molecular mass of 20.9kDa.
EPO a is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
EPO a protein solution (1mg/ml) contains 20mM Tris-HCl
buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSAPPRLIC DSRVLERYLL EAKEAENITT GCAEHCSLNE NITVPDTKVN
FYAWKRMEVG QQAVEVWQGL ALLSEAVLRG QALLVNSSQP WEPLQLHVDK AVSGLRSLTT LLRALRAQKE
AISPPDAASA APLRTITADT FRKLFRVYSN FLRGKLKLYT GEACRTGDR.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
