Catalogue number
PRO-2225
Synonyms
DHLAG, HLADG, Ia-GAMMA, li, HLA-DR antigens-associated
invariant chain, p33, Ia antigen-associated invariant chain, HLA class II
histocompatibility antigen gamma chain, CD74, Gamma Chain Of Class II Antigens,
MHC HLA-DR Gamma Chain.
Introduction
CD74 (HLA class II histocompatibility antigen gamma chain isoform b), is a transmembrane protein (on antigen presenting cells) which is the invariant chain that chaperones MHC class IIdimers from the endoplasmic reticulum to the cell surface. CD74 is expressed by cells of both T lymphocyte and Blymphocyte lineages. CD74 upregulated in several cancers and is also expressed by non immune cells during inflammation. Diseases related with CD74 include thymic epithelial neoplasm and gastroduodenitis. Among its related super-pathways are Immune System and Immune response Antigen presentation by MHC class II. In addition CD74 functions as cell surface receptor for the cytokine MIF.?
Description
CD74 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 166 amino acids (73-232a.a.) and
having a molecular mass of 19.05kDa (Molecular size on SDS-PAGE will appear at
approximately 18-28kDa). CD74 is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile filtered colorless solution.
Formulation
CD74 protein solution (0.5mg/ml) contains phosphate
buffered saline (pH7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
QQQGRLDKLT VTSQNLQLEN LRMKLPKPPK PVSKMRMATP LLMQALPMGA
LPQGPMQNAT KYGNMTEDHV MHLLQNADPL KVYPPLKGSF PENLRHLKNT METIDWKVFE SWMHHWLLFE
MSRHSLEQKP TDAPPKESLE LEDPSSGLGV TKQDLGPVPM HHHHHH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
