Catalogue number
PRO-2358
Synonyms
CD34 molecule, Hematopoietic progenitor cell antigen CD34, CD34 Antigen, CD34.
Introduction
CD34 is a single chain transmembrane glycoprotein which is selectively expressed on human lymphoid and myeloid hemapoietic progenitor cells. CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Description
CD34 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 268 amino acids (32-290a.a.) and having a molecular mass of 28.6kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
CD34 is fused with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile filtered colorless solution.
Formulation
CD34 protein solution (0.25mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
ADLSLDNNGT ATPELPTQGT FSNVSTNVSY QETTTPSTLG STSLHPVSQH GNEATTNITE TTVKFTSTSV ITSVYGNTNS SVQSQTSVIS TVFTTPANVS TPETTLKPSL SPGNVSDLST TSTSLATSPT KPYTSSSPIL SDIKAEIKCS GIREVKLTQG ICLEQNKTSS CAEFKKDRGE GLARVLCGEE QADADAGAQV CSLLLAQSEV RPQCLLLVLA NRTEISSKLQ LMKKHQSDLK KLGILDFTEQ DVASHQSYSQ KTHHHHHH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
