Catalogue number
CYT-961
Synonyms
Placental Growth Factor, Placental Growth Factor Vascular Endothelial Growth Factor-Related Protein, PGFL, PLGF, Placental Growth Factor-Like, Placenta Growth Factor, SHGC-10760, D12S1900, PlGF-2, PGF.
Introduction
PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It binds to receptor vegfr-1/flt1.
Description
PLGF3 Human Recombinant produced in Spodoptera frugiperda is a glycosylated homodimer containing 2 chains of 203 amino acids (Leu19-Arg221) and having a molecular mass of 58kDa.
The PLGF-3 is purified by proprietary chromatographic techniques.
Source
Spodoptera frugiperda.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
PLGF-3 protein was lyophilized from a 0.2μm filtered solution in HCl.
Solubility
It is recommended to reconstitute the lyophilized PLGF3 in sterile 4mM HCl not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized PLGF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF-3 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
The ED50, as measured by its binding ability to rHuVEGF R1 in a functional ELISA, is less than 3ng/ml.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
