Catalogue number
PRO-1423
Synonyms
F52, MACMARCKS, MLP, MLP1, MRP, MARCKS-related protein, MARCKS-like protein 1, Macrophage myristoylated alanine-rich C kinase substrate, MARCKSL1.
Introduction
MARCKS-related protein (MARCKSL1) which is a part of MARCKS family of PKC substrate is broadly used in the signal transduction studies as an indicator of PKC activation. MARCKSL1 plays a part in the coordination of membrane-cytoskeletal signaling events, including secretion, migration, phagocytosis and cell adhesion and is expressed in a variety of tissues with highest levels found in testis and uterus. Furthermore, MARCKSL1 serves as a regulator of Integrin activation and is thought to regulate Integrin-dependent signal transduction pathways, particularly those involved in macrophage spreading.
Description
MARCKSL1 Human Recombinant produced in E. coli is a single polypeptide chain containing 218 amino acids (1-195) and having a molecular mass of 21.9kDa. MARCKSL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The MARCKSL1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMGSQSSK APRGDVTAEE AAGASPAKAN GQENGHVKSN GDLSPKGEGE SPPVNGTDEA AGATGDAIEP APPSQGAEAK GEVPPKETPK KKKKFSFKKP FKLSGLSFKR NRKEGGGDSS ASSPTEEEQE QGEIGACSDE GTAQEGKAAA TPESQEPQAK GAEASAASEE EAGPQATEPS TPSGPESGPT PASAEQNE.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
