Catalogue number
PRO-1469
Synonyms
High Mobility Group AT-Hook 2, HMGIC, High-Mobility Group
(Nonhistone Chromosomal) Protein Isoform I-C, High Mobility Group AT-Hook
Protein, BABL, HMGI-C, LIPO, STQTL9, High Mobility Group Protein HMGI-C,
HMGA2.
Introduction
High Mobility Group AT-Hook 2 (HMGA2) is a member of the non-histone chromosomal high mobility group (HMG) family. HMG proteins perform as architectural factors and are necessary components of the enhancesome. This protein contains structural DNA-binding domains and may function as a transcriptional regulating factor. In addition, identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma proposes a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is implicated in diet-induced obesit. Among the Diseases associated with HMGA2 are diffuse lipomatosis, and 12q14 microdeletion syndrome.
Description
HMGA2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 117 amino acids (1-109 a.a) and having a molecular mass of 12.8kDa.
HMGA2 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
HMGA2 protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol, 2mM DTT and 250mM Imidazole.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MSARGEGAGQ PSTSAQGQPA APAPQKRGRG RPRKQQQEPT GEPSPKRPRG RPKGSKNKSP SKAAQKKAEA TGEKRPRGRP RKWPQQVVQK KPAQEETEET SSQESAEEDL EHHHHHH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
