Catalogue number
PRO-1212
Synonyms
SEC22 Vesicle Trafficking Protein Homolog B (S. Cerevisiae) Gene/Pseudogene, SEC22 Vesicle Trafficking Protein-Like 1 (S. Cerevisiae), ER-Golgi SNARE Of 24 KDa, SEC22L1, ERS-24.
Introduction
Vesicle-trafficking protein SEC22b precursor (SEC22B) is a member of the synaptobrevin family. SEC22B forms a complex with SNARE and it is believed to have a role in the ER-Golgi protein trafficking. SEC22B has a strong similarity to the mouse and Chinese hamster proteins.
Description
SEC22B Human Recombinant produced in E. coli is a single polypeptide chain containing 205 amino acids (14-194) and having a molecular mass of 23.3 kDa.
SEC22B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The SEC22B solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 2M Urea, 0.15M NaCl and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSM LPLAAS MQEDEQSGRD LQQYQSQAKQ LFRKLNEQSP TRCTLEAGAM TFHYIIEQGV CYLVLCEAAF PKKLAFAYLE DLHSEFDEQH GKKVPTVSRP YSFIEFDTFI QKTKKLYIDS RARRNLGSIN TELQDVQRIM VANIEEVLQR GEALSALDSK ANNLSSLSKK YRQDAKYLNM RSTYA
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
