Catalogue number
PRO-2187
Synonyms
Leucine Rich Repeat And Ig Domain Containing 1, LRRN6A, Leucine-Rich
Repeat And Immunoglobulin Domain-Containing Protein 1, Leucine-Rich Repeat
Neuronal Protein 1,
Leucine Rich Repeat Neuronal 6A, LERN1, Leucine-Rich
Repeat And Immunoglobulin-Like Domain-Containing Nogo Receptor-Interacting
Protein 1, Leucine-Rich Repeat Neuronal Protein 6A, UNQ201, LERN1, Leucine-rich
repeat and immunoglobulin-like domain-containing nogo receptor-interacting
protein 1.
Introduction
Leucine Rich Repeat And Ig Domain Containing 1, also known as Lingo1 is
primarily expressed in neuronal tissue, and most abundantly in the cortex. In
addition, Lingo 1 is involved in the inhibition of axon regeneration all the
way through a ternary complex formed with NgR1 (ligand-binding subunit) and p75
(signal transducing subunit). The inhibitory action is accomplished through
RhoA-GTP upregulation in response to the presence of MOG, MAG or Nogo-66 in the
central nervous system. Furthermore, LINGO-1 inhibits oligodendrocyte precursor
differentiation as well as myelination, by a mechanism which also involves
activation of RhoA, however it appears that it does not require 75 or NgR1.
Description
LINGO1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 133 amino acids (241-337 a.a) and
having a molecular mass of 15.1kDa.
LINGO1 is fused to a 36 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
LINGO1 protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSLKVL EISHWPYLDT MTPNCLYGLN LTSLSITHCN LTAVPYLAVR
HLVYLRFLNL SYNPISTIEG SMLHELLRLQ EIQLVGGQLA VVEPYAFRGL NYL.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
