Catalogue number
HSP-051
Synonyms
Heat shock protein beta-9, HspB9, Cancer/testis antigen 51, CT51, HSPB9.
Introduction
Heat Shock Protein B9 (HSPB9) is a member of the small heat shock protein (HSP20) family. HSPB9 is found in the cytoplasm as well as in the nucleus. HSPB9 is expressed specifically in the testis.
Description
HSPB9 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 179 amino acids (1-159 a.a.) and having a molecular mass of 19.6kDa.
HSPB9 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The HSPB9 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 50% glycerol, 0.2M NaCl and 2mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MQRVGNTFSN ESRVASRCPS VGLAERNRVA TMPVRLLRDS PAAQEDNDHA RDGFQMKLDA HGFAPEELVV QVDGQWLMVT GQQQLDVRDP ERVSYRMSQK VHRKMLPSNL SPTAMTCCLT PSGQLWVRGQ CVALALPEAQ TGPSPRLGSL GSKASNLTR.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
