Catalogue number
PRO-1757
Synonyms
Four And A Half LIM Domains 3, SLIM2, Skeletal Muscle
LIM-Protein 2, FHL-3, SLIM-2, Four And A Half LIM Domains Protein 3, LIM-Only
Protein FHL3.
Introduction
Four
and a half LIM domains 3 (FHL3) belongs to a family of proteins containing a
four-and-a-half LIM domain, which is a highly conserved double zinc finger
motif. FHL3 has been shown to interact with the cancer developmental regulators
SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the
high-affinity IgE beta chain regulator MZF-1. FHL3 is involved in tumor
suppression, repression of MyoD expression, and repression of IgE receptor
expression. Two transcript variants encoding different isoforms have been found
for this gene.
Description
FHL3 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 303 amino acids (1-280 a.a) and
having a molecular mass of 33.6kDa.?
FHL3 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
FHL3 protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSESFDC AKCNESLYGR KYIQTDSGPY CVPCYDNTFA NTCAECQQLI GHDSRELFYE DRHFHEGCFR CCRCQRSLAD EPFTCQDSEL LCNDCYCSAF SSQCSACGET VMPGSRKLEY GGQTWHEHCF LCSGCEQPLG SRSFVPDKGA HYCVPCYENK FAPRCARCSK TLTQGGVTYR DQPWHRECLV CTGCQTPLAG QQFTSRDEDP YCVACFGELF APKCSSCKRP IVGLGGGKYV SFEDRHWHHN CFSCARCSTS LVGQGFVPDG DQVLCQGCSQ AGP.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
