Catalogue number
PRO-1445
Synonyms
Golgin subfamily A member 7, Golgi complex-associated protein of 16 kDa, GOLGA7, GCP16, HDCKB03P, GOLGA3AP1, GOLGA7A, HSPC041, golgin A7.
Introduction
GOLGA7 is part of the ERF4 family. This protein is involved in protein transport from Golgi to cell surface. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS. GOLGA7 is palmitoylated on Cys-69 and Cys-72; that is required for Golgi localization and interaction with GOLGA3.
Description
GOLGA7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 160 amino acids (1-137 a.a) and having a molecular mass of 18kDa.
GOLGA7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
GOLGA7 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMRPQQAP VSGKVFIQRD YSSGTRCQFQ TKFPAELENR IDRQQFEETV RTLNNLYAEA EKLGGQSYLE GCLACLTAYT IFLCMETHYE KVLKKVSKYI QEQNEKIYAP QGLLLTDPIE RGLRVIEITI YEDRGMSSGR.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
