Catalogue number
PRO-2070
Synonyms
Guanine Nucleotide Binding Protein (G Protein), Q Polypeptide,
Guanine Nucleotide-Binding Protein Alpha-Q, CMC1, SWS, GAQ, Guanine
Nucleotide-Binding Protein G(Q) Subunit Alpha, G-ALPHA-Q, Guanine
nucleotide-binding protein G(q) subunit alpha.
Introduction
GNAQ, also known as Guanine nucleotide-binding protein belong to the G-alpha
family. Guanine nucleotide-binding proteins (G proteins) are involved as
modulators or transducers in a variety of transmembrane signaling systems. GNAQ
regulates B-cell selection and survival and is essential in order to prevent
B-cell-dependent autoimmunity. GNAQ also regulates chemotaxis of BM-derived neutrophils
and dendritic cells, in vitro. GNAQ is an alpha subunit in the Gq class,
couples a seven-transmembrane domain receptor to activation of
phospolipase C-beta. Mutations at this locus have been connected
with problems in platelet activation and aggregation. A related pseudogene to
GNAQ exists on chromosome 2.
Description
GNAQ Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 382 amino acids (1-359 a.a) and
having a molecular mass of 44.5kDa.
GNAQ is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
GNAQ protein solution (0.5mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.4M UREA and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMTLESIM ACCLSEEAKE ARRINDEIER QLRRDKRDAR RELKLLLLGT
GESGKSTFIK QMRIIHGSGY SDEDKRGFTK LVYQNIFTAM QAMIRAMDTL KIPYKYEHNK AHAQLVREVD
VEKVSAFENP YVDAIKSLWN DPGIQECYDR RREYQLSDST
KYYLNDLDRV ADPAYLPTQQ DVLRVRVPTT GIIEYPFDLQ SVIFRMVDVG
GQRSERRKWI HCFENVTSIM FLVALSEYDQ VLVESDNENR MEESKALFRT IITYPWFQNS SVILFLNKKD
LLEEKIMYSH LVDYFPEYDG PQRDAQAARE FILKMFVDLN PDSDKIIYSH FTCATDTENI
RFVFAAVKDT ILQLNLKEYN LV.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
