Catalogue number
PRO-999
Synonyms
Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62, p62, IBSN, SNDI.
Introduction
Nucleopurin 62kDa (NUP62) belongs to the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. Nucleoporins are the principal components of the nuclear pore complex in eukaryotic cells. This complex is a colossal structure which extends across the nuclear envelope, forming an entryway that regulates the stream of macromolecules between the nucleus and the cytoplasm. NUP62 associates with the importin alpha/beta complex that is involved in the import of proteins containing nuclear localization signals. Defects in the NUP62 are the cause of SNDI (infantile striatonigral degeneration), aka infantile bilateral striatal necrosis (IBSN) or familial striatal degeneration.
Description
NUP62 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 54,626 Dalton.
NUP62 is expressed with a -10x His tag at N-terminus and purified by proprietary chromatographic techniques.
Source
Sf9 insect cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
NUP62 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80% as determined by SDS-PAGE.
Coating concentration
0.5-1.0 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
Applications
Western blot with:
a) Polyclonal anti-Nup62 antibodies.
b) Monoclonal anti-hexa-His-tag antibody.
Immunological functions
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
