Catalogue number
PRO-1588
Synonyms
Gastrokine-2, Blottin, Down-regulated in gastric cancer, Trefoil factor interactions(z) 1, GKN2, BLOT, GDDR, TFIZ1, PRO813, BRICD1B, VLTI465.
Introduction
Gastrokine-2 (GKN2) is expressed in the gastric mucosa. GKN2 forms a heterodimer with TFF1- disulfide linked. GKN2 interacts with TFF2.
Description
Gastrokine 2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 21-184) containing 174 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 19.6kDa (calculated).
Source
Escherichia Coli.
Physical Appearance
Filtered White lyophilized (freeze-dried) powder.
Formulation
GKN2 filtered (0.4 μm) and lyophilized from 0.5mg/ml in 0.05M Acetate buffer pH-4.0.
Solubility
It is recommended to add 0.1M Acetate buffer pH-4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely at 37°C. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. GKN2 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MKHHHHHHASYEVFNIISPS NNGGNVQETV TIDNEKNTAI INIHAGSCSS TTIFDYKHGY IASRVLSRRA CFILKMDHQN IPPLNNLQWY IYEKQALDNM FSSKYTWVKY NPLESLIKDV DWFLLGSPIE KLCKHIPLYK GEVVENTHNV GAGGCAKAGL LGILGISICA DIHV.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
