Catalogue number
PRO-2065
Synonyms
Late Endosomal/Lysosomal Adaptor, MAPK And MTOR Activator
4, C7orf59, Late Endosomal/Lysosomal Adaptor And MAPK And MTOR Activator 4, Chromosome
7 Open Reading Frame 59, Ragulator Complex Protein LAMTOR4, UPF0539 Protein
C7orf59, LAMTOR4.
Introduction
LAMTOR4 is implicated in amino acid sensing and activation of mTORC1,
which is a signaling complex promoting cell growth in response to growth
factors, energy levels, and amino acids. LAMTOR4 is activated by amino acids via
a mechanism which involves the lysosomal V-ATPase, the Ragulator functions as a
guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator
and Rag GTPases both function as a scaffold recruiting mTORC1 to lysosomes
where it is sequentially activated.
Description
LAMTOR4 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 122 amino acids (1-99 a.a) and
having a molecular mass of 13.1kDa.
LAMTOR4 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
LAMTOR4 protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMTSALTQ GLERIPDQLG YLVLSEGAVL ASSGDLENDE QAASAISELV
STACGFRLHR GMNVPFKRLS VVFGEHTLLV TVSGQRVFVV KRQNRGREPI DV.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
