Catalogue number
PRO-1778
Synonyms
BEN Domain Containing 6, C6orf65, Chromosome 6 Open Reading Frame 65, bA203B9.1, BEN Domain-Containing Protein 6, BEND6.
Introduction
BEN domain-containing protein 6 (BEND6) is a neural BEN-solo factor which shares many functional attributes with Drosophila Insensitive, a co-repressor for the Drosophila CSL factor. The BEND6 protein binds the mammalian CSL protein CBF1 and antagonizes Notch-dependent target activation. Furthermore, its association with Notch- and CBF1-regulated enhancers is stimulated by CBF1 and antagonized by activated Notch. In utero electroporation experiments demonstrated that ectopic BEND6 impeded Notch-mediated self-renewal of neocortical neural stem cells and promoted neurogenesis.
Description
BEND6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 302 amino acids (1-279 a.a) and having a molecular mass of 33.6kDa.
BEND6 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
BEND6 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.1M NaCl.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMQKIVQT DEITNTQAFR KGKRKRTETM DSENANSDMD KGQRDPYSGN AFLPGESSSE DEEPLAELSK EELCAKIKSL KEKLTNTRKE NSRLRQSLVM LQVLPQAVTQ FEELVGMAEA LLKGGGTMST SASTLWRATN NSSPDSFAST CSNSNSNSSS PVSLKPEEEH QTDEKQFQIE KWQIARCNKS KPQKFINDLM QVLYTNEYMA THSLTGAKSS TSRDKAVKPA MNQNEVQEII GVTKQLFPNT DDVSIRRMIG QKLNNCTKKP NLSKNLNSQD IK.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
