Catalogue number
PRO-2379
Synonyms
EPHB2, CAPB, DRT, EK5, EPHT3, ERK, Hek5, PCBC, Tyro5, Developmentally-regulated
Eph-related tyrosine kinase, ELK-related tyrosine kinase, EPH tyrosine kinase
3, EPH-like kinase 5, hEK5, Renal carcinoma antigen NY-REN-47, Tyrosine-protein
kinase TYRO5, Tyrosine-protein kinase receptor EPH-3.
Introduction
EPH
Receptor B2 (EPHB2) is a part of the transmembrane Eph receptor tyrosine kinase
family (RTKs) which binds proteins of the Ephrin family on adjacent cells. The
interaction leads to contact-dependent bidirectional signaling into neighboring
cells. Hippocampal neurons can release vesicles containing full length
EPHB2, and these are taken up by neighboring glial cells. EPHB2 takes part in
the guidance of commissural axons through the embryonic midline and regulates
dendritic spines development and maturation and stimulates the formation of
excitatory synapses.
Description
EPHB2 Human Recombinant produced in Sf9 Baculovirus cells
is a single, glycosylated polypeptide chain containing 533 amino acids (19-543a.a)
and having a molecular mass of 59.1kDa (Molecular size on SDS-PAGE will appear
at approximately 50-70kDa). EPHB2 is fused to a 8 amino acid His-tag at C-terminus
& purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
EPHB2 protein solution (0.5mg/ml) containing Phosphate
Buffered Saline (pH 7.4), 1mM DTT and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
VEETLMDSTT ATAELGWMVH PPSGWEEVSG YDENMNTIRT YQVCNVFESS
QNNWLRTKFI RRRGAHRIHV EMKFSVRDCS SIPSVPGSCK ETFNLYYYEA DFDSATKTFP NWMENPWVKV
DTIAADESFS QVDLGGRVMK INTEVRSFGP VSRSGFYLAF QDYGGCMSLI AVRVFYRKCP RIIQNGAIFQ
ETLSGAESTS LVAARGSCIA NAEEVDVPIK LYCNGDGEWL VPIGRCMCKA GFEAVENGTV CRGCPSGTFK
ANQGDEACTH CPINSRTTSE GATNCVCRNG YYRADLDPLD MPCTTIPSAP QAVISSVNET SLMLEWTPPR
DSGGREDLVY NIICKSCGSG RGACTRCGDN VQYAPRQLGL TEPRIYISDL LAHTQYTFEI QAVNGVTDQS
PFSPQFASVN ITTNQAAPSA VSIMHQVSRT VDSITLSWSQ PDQPNGVILD YELQYYEKEL SEYNATAIKS
PTNTVTVQGL KAGAIYVFQV RARTVAGYGR YSGKMYFQTM TEAEYQTSIQ EKLPLLEHHH HHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
