RM199 specifically reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with unmodified Lysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM199 at 0.2 ug/mL, showed a band of Histone H4 acetylated at Lysine 5 in HeLa cells.
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody (clone RM199) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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