RM150 specifically reacts to Histone H3 monomethy- lated at Lysine 9 (K9me1). No cross reactivity with non- modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM150 at 0.5 ug/mL, showed a band of histone H3 monomethy- lated at Lysine 9 (K9me1) in HeLa cells.
ChIP performed on HeLa cells using H3K9me1 antibody (RM150, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Monomethyl-Histone H3 (Lys9) Rabbit mAb RM150 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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