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Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM135

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Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM135

品牌：RevMAb

貨號：31-1037-00

規(guī)格：100 ug

貨期：電詢

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Product Details
Product Name	Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM135]
Product Description	Rabbit monoclonal to Dimethylated Histone H3 Lysine 4, H3K4me2, Histone H3 (dimethyl K4)
Catalog No.	31-1037-00
Clone Name	RM135
Product Data Sheet	Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM135
Specificity	RM135 reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.
Immunogen	A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys4).
Application	Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.02 ug/mL – 0.2 ug/mL; ICC: 1 ug/mL - 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
	RM135 specifically reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with mono- methylated Lysine 4 (K4me1), trimethylated Lysine 4(K4me3), or other methylations in histone H3.
	Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM135 at 0.025 ug/mL, showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells.
	Immunocytochemistry of HeLa cells treated withsodium butyrate, using Dimethyl-Histone H3 (Lys4) Rabbit mAb RM135 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).