RM222 specifically reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.
Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using RM222 at 0.5 ug/mL, showed a band of histone H2A.Z acetylated at Lysine 7 in treated HeLa.
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H2A.Z (Lys7) Rabbit Monoclonal Antibody (clone RM222) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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