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ELISA of mouse immunoglobulins shows RMG07
reacts to mouse IgG1, IgG2a, IgG2b, and IgG3; no
cross reactivity with IgM, IgA, IgE, human IgG, rat IgG,
and rabbit IgG. The plate was coated with 50 ng/well
of different immunoglobulins. 500 ng/mL, 200 ng/mL,
or 50 ng/mL of RMG07 was used as the primary antibody.
An alkaline phosphatase conjugated anti-goat IgG as the
secondary antibody.